Intuitive procedure to assist you decide on the best chromatography column on your biopharmaceutical applications.
Conductivity detectors are bulk assets detectors considering the fact that They may be used to find out conductivity, and in their principal options is superior-sensitivity detection of billed species and surfactants (1).
Thanks to its substantial sensitivity, the fluorescence detector is particularly helpful for trace Evaluation, or when possibly the sample dimension is compact or the solute concentration is extremely very low.
The typical spectral bandwidth is 5–eight nm for the UV-vis detector. Since the Father sees all the spectrum, the spectral bandwidth is often specified from just one nanometer to any section of your entire spectrum through the control application.
When only solvent is passing with the sample component the measured refractive index of both equally elements is the same, but when an analyte passes in the stream mobile the two calculated refractive index are different.
A refractive index is a dimensionless amount describing how fast mild propagates by way of a medium when compared to the vacuum. Snell's law defines the refraction of light since it crosses the border between media with different refractive indices.
Write-up-column application of inverse gradient compensation gets rid of this impact by making certain the eluent moving into the detector has the precise solvent composition throughout the entire gradient separation.
Perfect for substantial-sensitivity measurements of molecules in intricate biological matrices where by analyte concentrations can span many orders of magnitude
The light that passes through the flow mobile enters the photodetector (photodiode) and is also transformed into an electrical sign comparable to the intensity of the light, that's processed as absorbance. A UV-seen detector which has a D2 lamp in addition to a tungsten lamp is appropriate for checking not merely ultraviolet and also obvious mild.
The here content here of our website is often available in English and partly in other languages. Select your most popular language and we will teach you the material in that language, if obtainable.
This page doesn't exist within your selected language. Your desire was saved and you'll be notified once a website page can be seen in your language.
HPLC lamps are light-weight resources designed for HPLC detectors. Agilent HPLC detector lamps are essential factors, in combination Using the optical device, and supply best depth, sensitivity, and security. Agilent HPLC lamps had been designed along with Agilent detector modules for optimum compatibility.
This web site won't exist as part of your picked language. Your choice was saved and you may be notified when a site could be seen as part of your language.
Chromatographers can use a few unique types of second-LC strategies to assistance enhance sample resolution by utilizing a number of column selectivity.